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Realistic waveforms can be synthesized using the empirical site amplification factor and phase models even though the source model is simple.
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Sites under positive selection (ω > 1) were estimated using the empirical Bayes methods in the site-specific and lineage specific analysis performed.
HyPhy was also used in functional constraint analyses among sites using the empirical Bayes technique, detailed results are in Additional file 10: Table S4.
The HyPhy analysis utilized the "MG94xHKY85x3_4x2_Rates" model of codon substitution [ 92] and searched for positively selected sites using the empirical Bayes method [ 6] following the procedure in [ 92].
In this study, the rate of evolution at each site was calculated using the empirical Bayesian paradigm that was shown to significantly improve the accuracy of conservation scores estimations over the Maximum Likelihood method, in particular when a small number of sequences was used for the calculations [ 53].
All the parameters were estimated using the empirical Bayes approach.
Amino acid sequences were simulated using the empirical JTT model.
The reliability of the empirical site response range was examined by elastic 1D linear numerical modeling using V s deduced from geophysical surveying and geotechnical boreholes.
Observations were recorded at KNK1 because the empirical site amplification factor was available for this site (Nozu et al. 2007), and the site was intended to be used as a reference site, as described in the following sections.
cPositively selected sites detected using the Bayes empirical approach implemented in the site models M8 and M2a.
Sites under selection were predicted using the Bayes Empirical Bayes (BEB) approach: sites inferred to be under positive selection with posterior probabilities >95% are in bold and sites with posterior probabilities of > 99% are indicated by an asterisk.
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