Sentence examples for using the emboss transeq from inspiring English sources

Sequences were translated to amino acids using the Emboss Transeq web application [ 87].

Sequences encompassing 100 nt upstream and downstream candidate SECIS elements identified by the covariance model were translated in full (including stop codons) using the EMBOSS transeq program and assuming UGA→U decoding.

Putative protein sequences were obtained by translating the cDNA sequences using the EMBOSS program "Transeq" [ 57].

ORFs were identified by using the EMBOSS software (version 5.0.0; emboss.sourceforge.net).net

Alignments of the parent genes and CG proteins were generated using the Emboss Needle program (http://emboss.sourceforge.net/) with default options.

GC content was calculated using the EMBOSS geecee program.

(G + C) content was calculated using the eMBOSS geecee program http://inn-temp.weizmann.ac.il/cgi-bin/emboss/geecee[ 63].

Sequence analysis was performed using the Emboss software suite [ 37], Clustal W [ 38] and 3DCoffee [ 39].

Introns were assigned by aligning the unigenes with the melon genomic sequence using the Emboss: est2genome [ 105].

Primer pairs were matched against bovine sequence from GenBank, NT and BTGI databases using the EMBOSS [ 26] primersearch tool.

The EMBOSS transeq program [ 53] was used to translate the C. intestinalis mRNAs into 6 possible reading frames.