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The GC contents of the 403 unitigs that had a hit against the P. infestans CDS were calculated using the EMBOSS tool Geecee [28] (Figure 3), as well as for all the 12,900 unitigs.
GC content was calculated using the EMBOSS tool geecee [ 63].
GC content was calculated using the EMBOSS tool geecee (Rice et al. 2000).
ORFs longer than 50 AAs between in-frame stop codons were extracted using the EMBOSS tool getorf [ 58].
Secondary protein structure was predicted using the Emboss tool Garnier [ 78], whilst the tertiary protein structure prediction and 3D modeling were performed using Phyre2 [ 37] and ESyPred [ 38].
Graphs of dN/dS ratios, HPD intervals and consensus sequences (generated using the EMBOSS tool cons [ 58]) were again generated using ggplot2.
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Primer pairs were matched against bovine sequence from GenBank, NT and BTGI databases using the EMBOSS [ 26] primersearch tool.
ORFs were identified by using the EMBOSS software (version 5.0.0; emboss.sourceforge.net).net
Alignments of the parent genes and CG proteins were generated using the Emboss Needle program (http://emboss.sourceforge.net/) with default options.
GC content was calculated using the EMBOSS geecee program.
(G + C) content was calculated using the eMBOSS geecee program http://inn-temp.weizmann.ac.il/cgi-bin/emboss/geecee[ 63].
More suggestions(15)
using the emboss server
using the emboss geecee
using the Trim tool
using the VDTNsim tool
using the Cochrane tool
using the emboss program
using the emboss dottup
using the WGAViewer tool
using the emboss package
using the emboss software
using the emboss sequence
using the emboss Water
using the MapMan tool
using the CloudMap tool
using the GOMiner tool
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