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Sequences were analyzed using the EMBOSS suite [ 27] and DnaSP [ 28].
Further confirmation of library sequences was performed using the EMBOSS suite of bioinformatics tools (http://emboss.bioinformatics.nl/).nl/
Sequence manipulation was carried out using the EMBOSS suite of programs [ 81].
Sequence manipulation was carried out using the EMBOSS suite of programmes [ 54].
Sequence manipulation was carried out using the EMBOSS suite of programmes [ 94].
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Sequence analysis was performed using the Emboss software suite [ 37], Clustal W [ 38] and 3DCoffee [ 39].
ORFs were identified by using the EMBOSS software (version 5.0.0; emboss.sourceforge.net).net
Intron/exon boundaries were identified by alignment of clover or legume orthologue cDNA with clover genomic sequence orthologues, or publicly available genomic legume databases such as M. truncatula or Lotus japonicus genomic sequence using the EMBOSS (European Molecular Biology Open Software Suite) est2genome programme.
Alignments of the parent genes and CG proteins were generated using the Emboss Needle program (http://emboss.sourceforge.net/) with default options.
GC content was calculated using the EMBOSS geecee program.
(G + C) content was calculated using the eMBOSS geecee program http://inn-temp.weizmann.ac.il/cgi-bin/emboss/geecee[ 63].
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