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ORFs were identified by using the EMBOSS software (version 5.0.0; emboss.sourceforge.net).net
The sites were mapped to 3 kb of promoter sequence of the β-catenin target genes using the Emboss program fuzznuc [31] allowing for 1 mismatch.
Alignments of the parent genes and CG proteins were generated using the Emboss Needle program (http://emboss.sourceforge.net/) with default options.
The presence of recognized DNA binding motifs and active sites was assessed by using the Expasy site and PFAM bioinformatics algorithms available at http://us.expasy.org/cgi-bin/protscale.pl, and the electrostatic charge was calculated by using the EMBOSS package (http://proteas.uio.no/EMBOSS).uio.no/EMBOSS
GC content was calculated using the EMBOSS geecee program.
(G + C) content was calculated using the eMBOSS geecee program http://inn-temp.weizmann.ac.il/cgi-bin/emboss/geecee[ 63].
Sequences were translated to amino acids using the Emboss Transeq web application [ 87].
Sequence analysis was performed using the Emboss software suite [ 37], Clustal W [ 38] and 3DCoffee [ 39].
Introns were assigned by aligning the unigenes with the melon genomic sequence using the Emboss: est2genome [ 105].
Primer pairs were matched against bovine sequence from GenBank, NT and BTGI databases using the EMBOSS [ 26] primersearch tool.
Secondary protein structure was predicted using the Emboss tool Garnier [ 78], whilst the tertiary protein structure prediction and 3D modeling were performed using Phyre2 [ 37] and ESyPred [ 38].
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