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Motor-domain sequences were extracted from their entire protein sequences by using the EMBOSS seqret utility [ 119] with base ranges obtained from SMART predictions.
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ORFs were identified by using the EMBOSS software (version 5.0.0; emboss.sourceforge.net).net
Alignments of the parent genes and CG proteins were generated using the Emboss Needle program (http://emboss.sourceforge.net/) with default options.
GC content was calculated using the EMBOSS geecee program.
(G + C) content was calculated using the eMBOSS geecee program http://inn-temp.weizmann.ac.il/cgi-bin/emboss/geecee[ 63].
Sequences were translated to amino acids using the Emboss Transeq web application [ 87].
Sequence manipulation was carried out using the EMBOSS suite of programmes [ 54].
Sequence analysis was performed using the Emboss software suite [ 37], Clustal W [ 38] and 3DCoffee [ 39].
Introns were assigned by aligning the unigenes with the melon genomic sequence using the Emboss: est2genome [ 105].
Primer pairs were matched against bovine sequence from GenBank, NT and BTGI databases using the EMBOSS [ 26] primersearch tool.
The corresponding chimpanzee cDNA and protein sequences were extracted and compared to the human ortholog by using the EMBOSS sequence analysis package.
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