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The ENVV2 DNA alignment similarity plot was created using the EMBOSS plotcon program [ 46] with a sliding window of size 32 and EDNAFULL as similarity matrix (figure 4A).
The quality of conservation of a sequence alignment of plant TPX2 domains was plotted using the EMBOSS plotcon software (Available from: http://bioweb.pasteur.fr/seqanal/interfaces/plotcon.html), with a standard window size of 4 for both DNA and protein sequences.
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ORFs were identified by using the EMBOSS software (version 5.0.0; emboss.sourceforge.net).net
Alignments of the parent genes and CG proteins were generated using the Emboss Needle program (http://emboss.sourceforge.net/) with default options.
GC content was calculated using the EMBOSS geecee program.
(G + C) content was calculated using the eMBOSS geecee program http://inn-temp.weizmann.ac.il/cgi-bin/emboss/geecee[ 63].
Sequence analysis was performed using the Emboss software suite [ 37], Clustal W [ 38] and 3DCoffee [ 39].
Sequences were translated to amino acids using the Emboss Transeq web application [ 87].
Introns were assigned by aligning the unigenes with the melon genomic sequence using the Emboss: est2genome [ 105].
Primer pairs were matched against bovine sequence from GenBank, NT and BTGI databases using the EMBOSS [ 26] primersearch tool.
Secondary protein structure was predicted using the Emboss tool Garnier [ 78], whilst the tertiary protein structure prediction and 3D modeling were performed using Phyre2 [ 37] and ESyPred [ 38].
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