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GC content was calculated using the EMBOSS geecee program.
(G + C) content was calculated using the eMBOSS geecee program http://inn-temp.weizmann.ac.il/cgi-bin/emboss/geecee[ 63].
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GC content was calculated using the EMBOSS tool geecee [ 63].
GC content was calculated using the EMBOSS tool geecee (Rice et al. 2000).
GC content was calculated using the EMBOSS tool geecee (Rice et al. 2000).For comparative analyses, reciprocal BlastN and TBlastXs searches between the genomes were carried out, leading to the identification of regions of similarity, insertions, and rearrangements.
Initial analysis of the genomic region included analysis of GC content using the EMBOSS [ 58] program "geecee" and analysis of repeats using RepeatMasker [ 59].
ORFs were identified by using the EMBOSS software (version 5.0.0; emboss.sourceforge.net).net
Alignments of the parent genes and CG proteins were generated using the Emboss Needle program (http://emboss.sourceforge.net/) with default options.
Sequences were translated to amino acids using the Emboss Transeq web application [ 87].
Sequence analysis was performed using the Emboss software suite [ 37], Clustal W [ 38] and 3DCoffee [ 39].
Sequence manipulation was carried out using the EMBOSS suite of programmes [ 54].
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