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The absorbance was measured at a λ of 450 ηm, using the ELISA reader.
The absorbance was read at 450 nm using the ELISA reader (Tecan, Seestrasse, Männedorf, Switzerland).
The absorbance was measured at a wavelength of 450 ηm using the ELISA reader.
The analysis was performed using the ELISA reader SUNRISE (Tecan, Salzburg, Austria) at a wavelength of 450 nm.
The reaction was stopped with 100 μl of 1 N sulfuric acid and measured using the ELISA reader at 450 nm.
About 10 minutes later, stop solution was added, and absorbance was measured using the ELISA reader at 450 nm with 630 nm as the reference wavelength.
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The absorbance of the plates was measured using the ELISA-reader α-Quant (Bio-Tek, Bad Friedrichshall, Germany).
Absorbances were recorded at 405 nm using the ELISA plate reader and amounts of converted substrate were read from a para-nitrophenol standard curve.
Absorbances were recorded at 560 nm using the ELISA plate reader and amounts of protein were read from a bovine serum albumin standard curve.
Fresh culture medium with 10% assay reagent was added, and after three-hour incubation absorbance values of the medium were read at 560 nm and 595 nm using the ELISA plate reader.
After 15 min, the amount of colored formazan derivative was determined by measuring optical density (OD) using the ELISA microplate reader (Biorad, Hercules, CA) at 570 nm (OD570-630 nm).
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