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Plasma cytokine and chemokine levels were determined using the ELISA methods described above, whereas the soluble form of CD62L was quantified using a specific kit (cat no. MLS00; R&D Systems).
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The standard curves were obtained using the ELISA method or the commercial BCA assay kit, according to the manufacture's instructions.
G6PD deficiency was defined as enzyme activity of less than the reference value (4 IU/gHb), as measured using the ELISA method [30].
Serum testosterone levels were obtained using the ELISA method [ 18].
Western blot data (data not shown) confirmed the observation obtained using the ELISA method as well.
Rheumatoid factor (RF) IgM was analyzed using the ELISA method [ 21].
Cortisol will be extracted from hair using the Elisa method [ 18].
In their more recent study, Kusaka et al. found that sNGAL measured using the ELISA method correlated well with sNGAL measured using the ARCHITECT method.
Based on this result, two dilutions of 1 1000 and 1 2000 were further verified using the ELISA method for the LP as described above.
The level of cytokines was measured using the ELISA method (BD OptEIA, BD Biosciences) in cell culture supernatants of macrophages treated with a noncytotoxic concentration of the tested fraction.
The serum ADMA levels were measured using the ELISA method (Catalog Number: AAP31-K02, Eagle Biosciences, Inc., Boston, MA, USA), according to the manufacturer's instructions.
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