Sentence examples for using the ecl method from inspiring English sources

Membranes were exposed using the ECL method (GE Healthcare) according to the manufacturer's instructions.

After incubation with the relative second antibody, immune complexes were detected using the ECL method.

Transfer was followed by blocking of the membrane (30' at RT in 5% dry milk TBS-T), incubation with primary antibody (overnight at 4°C, in 0.5% BSA TBS-T), secondary antibody (2 h at RT, in 1% dry milk TBS-T), results were revealed using the ECL method (SuperSignal West Chemiluminescent Substrate - Pierce, Hyperfilm ECL - Amersham).

Proteins were detected using the ECL method.

The blots were developed using the ECL method (Amersham).

The blots were developed using the ECL method (GE Healthcare).

Following incubation in an anti-rabbit HRP conjugated secondary antibody (Dako, Glostrup, Denmark; 1 2,500) for 1 h at room temperature, proteins were detected using the ECL detection method.

Immunoreactive bands were visualized by using the ECL (enhanced chemiluminescence) detection method (Amersham, Arlington Heights, IL, USA) (Gong et al, 1995).

The proteins were visualized by a horseradish peroxidase method using the ECL kit from Kirkegaard and Perry Laboratories Inc., Gaithersburg, Maryland, USA.

The blots were developed using the ECL-plus detection method (Amersham, Sweden).

Each blot was developed using the ECL-plus detection method (Amersham) and quantified using ImageQuant Las 4000 mini gold (GE Healthcare Bio-Sicences).