We measured hourly total PM2.5 OC and elemental carbon (EC) using the OC_EC Analyzer (model 3F; Sunset Laboratory Inc., Tigard, OR, USA) and then estimated exposure to carbonaceous aerosol classes characterized by primary and secondary sources using the "EC tracer method" (Cabada et al 2004; Polidori et al. 2007).
Finally, immunolabeled protein bands were detected using the ECL(Electro-Chemi-Luminescence) method (Immobilon™ Western, Millipore Corporation, Billerica, MA, USA) and quantified using an Alpha Imager 2200 (ProteinSimple, Santa Clara, CA, USA).
Thus, in our study, it is a risk to use the EC residual method to estimate LE since there were more uncertainties from Rn, G, S, and other heat sinks, especially when LE is smaller or under a certain spatial scale.
Re-analysis of our previous data, using the same EC methods as those used here, showed that, by contrast, objIMG shared more common paths with fHS than uHS.
Protein Interactions and the EC method group all pathways together.
Fig. 9 Illustration of mesh patching technique: a original mesh with the highlighted patch of the point to be removed; b mesh with polygon of the empty patch; c mesh patching using constrained Delaunay triangulation (CDT) for b; d mesh patching using ear clipping (EC) method for b.
The activity of trypsin (EC 3.4.21.1) and chymotrypsin (EC 3.4.21.2) were measured using the modified method reported by Lainé et al [ 16] and the activity of α-amylase (EC 3.2.1.1) was assayed according to Bernfeld [ 17].
Using the method described here, EC readings of 4 or higher could indicate danger.
The most commonly used EC method in developing countries including Ghana is the ECPs.
Signals were detected on FUJI Super RX films (Fujifilm, www.fujifilm.com) using the enhanced chemiluminescence method (GE Healthcare, ECL™ Western Blotting Analysis System).
