We decided to use the 2005 expression data for the cluster representation (Fig. 4), due to the smaller variance among replicates [see Additional file 2].
The statistical analysis was performed using the REST 2009 (Relative Expression Software Tool - Qiagen, Inc).
Also we used the KIAA0101 prokaryotic expression for the test of its antibody specificity.
To determine if Kv4 function in motoneurons contributes to this defect, we used the c164-Gal4 toansgene to drive expression of UAS-DNKv4 only in motoneurons.
Hereafter, we use the phrase metabolic-rate-adult-equivalent wolves to indicate pack size was determined while using the expression (masspup3/4/massadult3/4). We calculated and compared per capita prey acquisition rates using each method for each month of summer (nMay = 6, nJune = 7, nJuly = 5).
Numerical results of the comparison, by using the expression (10), were summarized in Table 1 (binarization obtained by recursively applying the mean shift filtering), Table 2 (binarization obtained by using graph), and Table 3 (binarization obtained via Otsu's method).
We used the 7897 genes derived from control gene expression described in the text as our background.
After digestion with restriction enzyme, pMD18-MPT64 was to pET21a using T4 ligase and the pET21a-MPT64 explasmidn plasmid was used to transform BL21 (DE3) competent cells.
An expression index (EI) was calculated for telomerase expression using the formula 1,000 × 2 (-ΔCt), where ΔCt = Ct (telomerase) - Ct (GAPDH).
The Ostwald-de Waele model (Power law) was used to describe the experimental shear dynamic viscosity data, η, as a function of the shear rate, γ, in the shear thinning region for each concentration of both sets of nanofluids by using the following expression [46 48]: η = K· γ ˙ n − 1, (7).
