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Bladder values are theoretical values derived using the dynamic bladder module described above.
These parameters were used to estimate the residence time for [18F]-tetrafluoroborate in the bladder using the dynamic bladder model in the OLINDA/EXM software program, with the assumption of a 2.4-h voiding interval.
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The residence time for the bladder was estimated using the Medical Internal Radiation Dose (MIRD) dynamic bladder model with a 2-h voiding interval and a single or biexponential fit of the remainder of the body (total minus all organs) plus bladder contents fraction of injected activity versus time as the input function.
However, the dose of the UB when using the International Commission on Radiological Protection (ICRP) dynamic bladder model is displayed in brackets for the human study in the "Results" and "Discussion" sections.
Urine excretion data were modeled using the implemented dynamic voiding bladder module [11] in OLINDA/EXM.
The NCA of the urinary bladder contents and voided urine was calculated from the analytical fit to the summed activities in the urinary bladder contents and voided urine using a dynamic urinary bladder model [9].
The urinary bladder contents' residence times were calculated using the OLINDA implementation of Cloutier's dynamic bladder model assuming a 4.8 h void schedule.
The parameters (fraction and associated biological removal half-time) of the corrected whole body time activity curve were used as input data for the dynamic (urinary) bladder model.
The gene expression relative to normal bladder RNA (human bladder total RNA, Clontech) was calculated using the comparative Ct method.
The time-integrated activity coefficient for urinary bladder content was calculated by applying the dynamic urinary bladder model [10] to the urine samples with a bladder voiding interval of 2 h.
The challenge is to reconstruct our model in a more physiological environment, with the use of a bioreactor that mimics the dynamic of bladder filling and emptying, to acquire physiological properties.
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