Sentence examples for using the double transgenic from inspiring English sources

Using the double transgenic zebrafish line Tg(flt1 YFP Tg kdr-l RFP), and morpholinos to knock down vascular endothelial growth flt1 YFP Tg kdr-l RFPhospholipand cγ1 (plcγ), the authors then dissected angiogenic sprouting and lymorpholinosic budding from toe posterior cardinal vein by in vivo imaging.

Supporting this conclusion, Sox-2 expression was not qualitatively affected upon a constitutive activation of Notch signaling by expressing the intracellular domain of Notch (NICD) by neuromast-specific chemical induction with tamoxifen using the double transgenic line Tg[Cldnb Gal4 ERT2;5xUAS-E1b:6xMYC-notch1a] (Fig. 4A-G).

Using the double-transgenic line Tg[Atoh1a:dTomato ; ET krt4 EGFP sqet4] to simultaneously visualize Atoh1a expression and hair cells, we extended these previous observations by finding a broad but weak Atoh1a in supporting cells, which stabilizes and becomes stronger in UHCPs and in young hair cells (Fig. 3E).

To investigate the influence of Schwann cells on the rate and extent of axon regrowth after injury, we used the double-transgenic larvae Tg[gSAGFF202A SILL mCherry] at 5 dpf (Fig. 6A), when the posterior lateralis ganglion contains around 50 neuronal perikarya.

Microarray analyses and reverse-transcription polymerase chain reaction were performed using liver samples of both the double transgenic mice (DTM), which express both the HCV core and tTA, and single transgenic mice (STM), which express tTA alone, at 2 months of age.

Before starting our study, we investigated the appropriate time-frame to assess an exogenous Aβ seeding effect in the double transgenic mouse model used in this study.

ΔΔCt values were derived by subtracting the ΔCt values of the wild type from those of the single transgenic and double transgenic mice, which were then used to calculate the levels of the mRNA in the double transgenic mice as a percentage of the level in the wild type mice.

Brain weight comparisons between the double transgenic and the wild type were done using Student's t-test (Table S1).

To characterize the effect of XIK1 on XA21-mediated disease resistance, we performed more detailed investigations using the plants (XXIK1Ri) homozygous for both Xa21 and XXIK1Ri derived respectively from three of the double transgenic lines.

The double transgenic plants carrying Xa21 and silenced for XIK1 are partially resistant, showing typical long water-soaked lesions.

The double transgenic UAS:dnpdgfrβ-yfp hsp70 Gal4 line was then crossednpdgfrβ-yfp hsp70 Gal4transgenic fline:eGFP;hsp70:Gal4 line to generate triple transgenic UAS:dnpdgfrβ-yfp;hsp70:Gal4;fli1a:eGFP embryos to be used for the experiment.