Exact(5)
Samples were taken at regular intervals and titrated immediately using the double agar overlay method.
The final titre of bacteriophages in the supernatant was determined using the double agar overlay method, as described above.
Phage counts were determined using the double agar method, while bacterial numbers were counted after plating a dilution series of the supernatant.
Coliphages were detected using the double agar layer method.
We analyzed coliphages using the double agar layer (DAL) method or enrichment method as described in U.S. EPA Methods 1601 and 1602 (U.S. EPA 2001a, 2001b).
Similar(55)
Bacteriophage stocks were prepared using the double-agar overlay method with minor modifications.
Samples were assayed to determine pfu using the double-agar layer technique [ 42].
Samples were assayed to determine surviving pfu using the double-agar layer technique [ 42].
The number of free phages in the filtrate was determined in duplicate, using the double-agar layer method.
The bacteriophage titer in the filtrate was assessed using the double-agar layer technique according to Adams (1959).
After all these experiments, the bacteriophage titer was assessed using the double-agar-layer technique (Adams 1959).
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