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Using the Distance software line transects were placed systematically with a random start within each survey stratum, using the "Systematic Segmented Trackline Sampling" design class within the automated design component of the software.
Secondly, placement of transects using the Distance software within the Toukoulaka FMU proved difficult because of its convoluted boundary between the terra firma forests to the west and the swamp forests to the east (Figure 1).
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The survey was designed and the results analyzed using the Distance 5.0 (release 2) software [74].
In the same scans, maximum orthogonal diameters were measured using the distance measurement tool in standard visualization software in axial and coronal sections.
The phylogenetic tree was generated with MEGA4.0 software [ 49] using the distance model for amino acid substitution of Jones-Taylor-Thornton (JTT) matrix, the Neighbor-joining algorithm for clustering and 1000 replications for the bootstrap analysis.
Phylogenetic analyses were conducted with the MEGA version 3.1 software (26 ) by using the distance method and the neighbor-joining algorithm with Kimura-2 parameters.
Combination of data sets: both data sets were combined using the software "Distance Weighted Discrimination" (https://genome.unc.edu/pubsup/dwd/) to obtain a collection of 174 samples (166 tumors) and 5319 common genes.
Obtained data were processed using the software package Distance Sampling 5.0 [35] which provided an estimation of the density of animals in each site [36].
Phylogenetic trees were constructed by neighbour joining from 100 samplings of maximum-composite-likelihood (MCL) distances using the MEGA 4.0 software package [47] with pairwise deletion for missing data.
Using the software "ruler", the distance from the knee to the top of the regenerate and the height of the regenerate were measured and recorded.
Light intensity and phase shift are recorded for each emitter-optode distance using the proprietary software package OxiTS, and the absolute μa and μs' are calculated based on the slope of the respective regression lines at each wavelength using the diffusion equation for homogeneous, semi-infinite media [ 1, 2].
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