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Birds were estimated using the distance sampling method [ 30].
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To estimate whale density, we conducted visual line transect surveys along four north-south and four east-west tracklines (i.e., long shore and perpendicular) using standard line-transect methods [22] and analyzed sighting data with Distance (V5.0), using the conventional distance sampling analysis engine [23].
Unbiased estimation of variance of Horvitz Thompson estimator is also possible using the distance balanced sampling plans.
We also perform a principle coordinate analysis using the distance matrices and plot the samples on the first two principle coordinates (Fig. 5).
Hence the distance obtained is not the distance projected on the sea surface, required for unbiased density estimation using a conventional distance sampling approach, but the three-dimensional slant distance with respect to the ship's track line.
Using line-transect distance sampling techniques, porpoise density and abundance were estimated for each sub-area and survey season.
Larger clusters are often easier to detect than smaller ones, leading to a potential bias when determining population mean cluster size; this is often dealt with using multiple covariate distance sampling (MCDS, see Marques & Buckland, 2003; Marques et al., 2007).
It uses the distances between samples to partition variance and randomizations or permutations of the data to produce the p-value for the hypothesis test.
We used distance sampling techniques to account for variability in detection probability to generate more reliable density estimates than unadjusted counts provide.
Similarities among individual sample profiles were calculated using the sample distance matrix.
This would allow reasonable survey designs for abundance estimation to be implemented and the data analysed using conventional distance sampling methods.
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