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Gene expression during various developmental stages and the differential expression responsible for parathion resistance were profiled using the digital gene expression (DGE) technique.
Using the digital gene signatures, more structure became apparent.
Sequence tag preparation was performed using the Digital Gene Expression Tag Profile Kit (Illumina) according to the manufacturer's instructions.
The four tag libraries of mandarin fish (the liver tissues of feeders and nonfeeders, the brain tissues of feeders and nonfeeders, respectively) were constructed in parallel using the Digital Gene Expression Tag Profile Kit (Illumina) according to the manufacturer's instructions.
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Here, we used the digital gene expression (DGE) method to perform a deep transcriptome analysis of bees fed with pollen.
In this study, we used the digital gene expression tag profiling (DGEP) strategy driven by high-throughput sequencing technology to profile the transcripts at different stages of P. patens gametophyte development and obtained spatiotemporal-specific gene regulation models for growth and differentiation.
These reads were used in the digital gene expression analysis of all 14 tissues.
Reads from the high quality category that mapped uniquely were used for the digital gene expression counts.
The number of qualified reads for each gene was tallied and normalized to TPM (number of transcripts per million qualified reads), which was then used as the digital gene expression abundance of the gene.
In this study [ 9], digital control has been measured using the digital CTC (Control Type Confidence), a measure very similar to our metabolic coherence (see Methods), that evaluates the coherence of patterns of differentially expressed genes with the TRN.
A lab-owned R program was then used to compute the digital gene expression profiling.
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