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PCR primers were designed successfully using the differential display sequence to develop a RT-PCR-based method to detect TSV.
To clarify the cause of abnormal behavior in PACAP deficient-mice, we attempted the identification of genes whose expression was altered in the dentate gyrus of PACAP-deficient mice using the differential display method.
To elucidate molecular events associated with PACAP deficiency in the mouse brain that could be relevant to schizophrenia, therefore we attempted to detect PACAP deficiency-regulated genes in the dentate gyrus using the differential display (DD) method and found that stathmin1 expression was up-regulated in the dentate gyrus of PACAP-deficient mice.
To find new genes we apply the concept of Expression Genetics (Sager, 1997), using the differential display method (Pardee, 1999).
The mRNA differential display was performed using the differential display kit from Eurogentec Belgiumm) and following the instructions of the supplier.
A previous study using the differential display technique showed higher expression of CRS4C in the small intestines of SPF ICR mice than of GF ICR mice [ 40].
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To identify IEGs, we used the differential display method to search for honeybee genes that are immediately induced in the brain by neural activity.
We used the differential display (DD) RT-PCR method using normal cervical, cervical cancer, metastatic cervical tissues, and cervical cancer cell lines to identify genes overexpressed in cervical cancers and identified gremlin 1 which was overexpressed in cervical cancers.
Total RNA extracted from the same clones (hTNF/TAg SF B2, wt/TAg SF F6) used for the differential display, grown under identical conditions, was used to hybridize the Mu11K (A,B) high-density oligonucleotide chip set from Affymetrix.
The RNA was used to synthesize the corresponding cDNA, which was subsequently used for the differential display PCR reaction.
Using the fluorescent differential display (FDD) technique, the differential expression pattern of genes in highly susceptible silkworm strain 306 were analyzed, following infection with B. mori nuclear polyhedrosis virus (BmNPV), and a differential band (G12782) was obtained from the hemolymph RNA pools.
More suggestions(15)
using the differential quadrature
using the differential equation
using the new display
using the differential method
using the differential inversion
using the differential training
using the differential Evolution
using the template display
using the flexible display
using the differential height
using the differential property
using the differential evolution
using the external display
using the phage display
using the differential transformation
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