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The test consists in using the different sequence patterns (Fig. 8C) individually to do homologue searching.
Using the different sequence databases available in amniotes, a long variant transcript, corresponding to a read-trough transcript of Nme1 and Nme2 genes can be found in human, chimpanzee, horse, cow, platypus, and anole lizard (Table 1).
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Additionally, TCF/LEF responsive elements, specific transcription factors associated with WNT signaling, were investigated using the different consensus sequences as previously identified [ 39].
To examine this hypothesis, phylogenetic analysis was performed using the different isolated SIDER sequences.
The results of our analyses of the three (partial) sequences using the different approaches outlined above are largely congruent, enabling us to derive general conclusions regarding the phylogeny, host switches, and biogeography of Yponomeuta.
This table shows the performance of RBH using the different BLAST filtering options with eukaryotic sequences.
We used the standard Illumina adaptors and primers used in the different sequencing assays as target sequences to be filtered out from the reads.
Out of the six Musaceae species evaluated using the two different sequencing approaches, only M. textilis gave polymorphic ITS sequence after direct Sanger sequencing.
No studies to our knowledge have evaluated the measures longitudinally nor have any studies compared the association of BMLs measured with the two different sequences as they relate with pain, which would provide insight into the construct validity of the BMLs measured using the two different sequences [ 7].
In addition to Sanger sequencing, we used massively parallel sequencing (454) to study the diversity of the ITS region within six diploid Musaceae accessions (Table S1) and to compare the data obtained using the two different sequencing approaches.
Rarefaction curves were estimated out of the distance matrix from the different sequence alignments using the program DOTUR and the conventional species cut-off limit (members of a same OTU have >97% SSU rDNA sequence identity) [48].
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