In many Gram negative bacteria, including Y. pestis, the type III secretion system (T3SS) is the preferred target for novel therapeutics development using the described strategies [2], [3], [4], [5], [6], [7], [8], [9].
We have also successfully used the described strategy to obtain an antibody against another related protein – mouse acetylcholinesterase (data not shown).
For numerosities of five or larger, it could become faster to use the described strategy than to judge the number of target items.
Using the described searching strategy above, a total of 549 articles were initially retrieved out of PubMed, Embase, the Cochrane Library, CNKI, and Wan Fang databases.
When a contact person was listed in ClinicalTrials.gov and no publication was identified using the described search strategy, a study author (CWJ) attempted to contact that investigator once by email to inquire if results had been published.
Sample preparation was identical for both PB and BM samples and run using the described sequential gating strategy.
White and opaque cells were easily distinguished microscopically and were counted separately using the described Cy3 antibody based strategy described in Materials and Methods.
By using the above described strategy, 53 publications have been retained for further analysis.
So far, there has no report using the above described strategy for gene deletion in Clostridium yet.
Primers for sFRP genes were selected using the previously described strategy (Merle et al, 2004).
