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Extraction and quantification of PCA from the culture suspension were performed using the described methods [48].
We could not detect anything in the 100% ethyl acetate fraction of C. uleana using the described methods, nor with HPLC-MS, nor with GC-MS after transmethylation to detect fatty acids.
We chose to present examples that we ourselves have presented in major clinical journals, including The BMJ EK and DR are experienced statisticians and health services researchers who have published numerous clinical papers using the described methods.
A key challenge in the incorporation of MD data in research studies or clinical practice is that the assessment of MD by using the described methods, when performed on a large scale, is heavily restricted because of time and cost.
Using the described methods, we were able to detect at least one known diarrheal pathogen in 75% of all diarrheal cases, which is comparable to the findings by previous case control studies conducted in the north of Vietnam (67%; N = 587), 23 and in seven developing countries in Africa and Asia within The Global Enteric Multicenter Study GEMSS) (83%; N = 9,439).
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Extraction of RNA and cDNA was carried out using the described method.
The performance of the control system using the described method compared well to results reported in literature.
However, the low level of inducible phosphorylation of Lck and Fyn made the immunoblots difficult to quantify using the described method.
Nineteen BoHV-5 field isolates, including two newly identified ones, were subtyped using the described method.
When using the described method on sediment samples, we found that the selected biomarker is present only in the upper part of the sediment core.
First; the ladderane PC lipid at m/ z 816 displays the highest abundance in the mass spectrum when using the described method.
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