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Quantification of the blots was performed using the densitometry feature of Photoshop.
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To determine the refractive outcomes of wavefront-optimized laser in situ keratomileusis (LASIK) treatments, in particular to measure corneal densitometry after LASIK using the densitometry function of the Pentacam Scheimpflug system.
The picture was then color-inverted and subjected to densitometry analysis by using the ImageJ densitometry software (Version 1.44, NIH).
The results were analyzed using the ImageJ densitometry system.
Quantification of protein bands (ECL signals) was performed by densitometry using the Quantity One densitometry software (Bio-Rad LaboratorieSwitzerlandch, Switzerland).
Immunoreactive products were visualized by chemiluminescence (SuperSignal Femto West, Pierce Biotechnology, Rockford, IL) and quantified by densitometry using the ImageJ digital densitometry software (http://rsbweb.nih.gov/ij/).
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Band intensities were measured using the Quantity One densitometry software package (Bio-Rad Lab, USA).
Immunoreactivity was quantified using the FluorChem spot densitometry software.
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