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For RNAi experiments, 0.75 million primary macrophages were transfected with nonspecific (NS) control or SMARTpool siRNAs (100 nM, Dharmacon) directed against Srf, Mkl1, Mkl2 and Elk4 using the Deliver X transfection reagent (Panomics) according to the manufacturer's instructions.
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In addition, oxygen saturation at the capillary end of well-perfused capillaries is low, suggesting that the tissues are using the delivered oxygen.
Using the delivered dose in our study should provide more accurate evaluation of the effect on daily solute control.
Our next goal was to investigate the possibility of using the delivered tandem monobody to perturb protein function and related signaling pathways in cancer cells.
The model uses the delivered feedstock cost, energy consumption, and GHG emissions as system performance criteria.
This means that anyone with a computer or smartphone and a Wi-Fi connection can use the Deliver Now feature.
Using the appropriate delivering catheters, procedure time can be decreased.
Since most perinatal outcomes can only occur in women and neonates who have delivered, using the number of pregnancies delivered (PD) as the denominator of outcomes is appropriate.
We are using the insights to deliver on consumers' Needs and Wants.
The advantages of using the Web to deliver virtual patients are however overwhelming.
However, there are also disadvantages of using the Internet to deliver health care interventions.
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