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Quantitative RT-PCR was carried out on an ABI 7500 using the default cycling parameters.
Reactions were run on ABI PRISM 7500 using the default cycling conditions.
Reactions were run on a CFX96 Real-Time System + C1000 Thermal Cycler (Biorad) using the default cycling conditions.
Reactions were run on ABI 7900 HT Sequence Detection Systems using the default cycling program, and data were processed using SDS 2.2 software (Applied Biosystems).
Reactions were run on the ABI 7500 Sequence Detection Systems using the default cycling program, and data were processed using SDS 2.2 software (Applied Biosystems).
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QPCR was performed with 20 ng gDNA according to the manufacturer's protocol in an Applied Biosystems One Step Plus Real-Time PCR System using the default universal cycling conditions.
Cycling was performed using the default conditions of ABI 7300 SDS Software 1.3.1: 2 min at 95°C, followed by 30 35 cycles of 15 sec at 95°C and 1 min at 60°C.
PCR cycling conditions were performed using the default conditions of the ABI Prism 7300 SDS Software.
The second keep using the default value of.
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Background signal was subtracted using the default rolling ball parameters.
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CEO of Professional Science Editing for Scientists @ prosciediting.com