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Categorization of genes on the list based upon Gene Ontology (GO) assignments were also done using the DAVID program and the outcome showed similar categories to those assigned under the KEGG Pathway (not shown).
For comprehensive annotation, all putative target genes in each sample were analyzed by GO terms using the DAVID program.
To identify the biological processes associated with the genes from the microarray, differentially expressed genes were interrogated using the DAVID program.
We then analysed the GO enrichment of these upregulated and downregulated proteins by using the DAVID program with X. laevis proteins and candidate human homologues.
To obtain a perspective on which processes are most shared across the DGAP experiments, we took the set of 520 genes differentially expressed in four or more data set and calculated the enrichment of Gene Ontology (GO) labels using the DAVID program (Dennis et al., 2003) with the results shown in Table 4.
Using the DAVID program, GO terms for biological process, molecular function and cellular component were associated with 75% (4,527) of the 6,061 ESTs that hit with an annotated protein in the NCBI database (Fig. 4 and see Additional files 1, 2 and 3).
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For annotation we used the DAVID program [ http://david.abcc.ncifcrf.gov].abcc.ncifcrf.gov]
To take an unbiased approach to the identification of other genes that might be involved in maintenance of the symbiosis, we used the DAVID program to identify biological processes (based on GO terms) that were significantly overrepresented among the differentially expressed transcripts (see Materials and Methods).
Functional annotation and enrichment analyses were done using the DAVID platform version 6.7 (http://david.abcc.ncifcrf.gov/home.jsp) [36], [37].
GO enrichment analysis was performed using the DAVID web accessible program [12,13] (http://david.abcc.ncifcrf.gov).abcc.ncifcrf.gov
A gene ontology (GO) analysis was performed using the DAVID website.
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using the david webtool
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using the david analysis
using the david data
using the david method
using the FigTree program
using the david gene
using the Neighbor program
using the david interface
using the david platform
using the david website
using the david formula
using the david software
using the david annotation
using the ImageJ program
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