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Functional annotation and enrichment analyses were done using the DAVID platform version 6.7 (http://david.abcc.ncifcrf.gov/home.jsp) [36], [37].
We completed a GO clustering analysis for each gene profile subset with > 1.5-fold change in expression during erythroid maturation using the DAVID platform [ 41].
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For annotation we used the DAVID program [ http://david.abcc.ncifcrf.gov].abcc.ncifcrf.gov]
GO enrichment analysis was performed using the DAVID web accessible program [12,13] (http://david.abcc.ncifcrf.gov).abcc.ncifcrf.gov
In each analysis we only used genes found on both platforms and then performed a pathway analysis using the DAVID software [ 7, 8].
For cross-platform comparison, Affymetrix probe sets and Codelink identifiers were mapped to Unigene ids using the DAVID annotation tool (see above).
A gene ontology (GO) analysis was performed using the DAVID website.
Using the DAVID functional annotation clustering tool, the up- and down-regulated genes can be categorized into 176 and 146 overlapping functional gene clusters, respectively.
Differentially expressed genes were categorized in GeneOntology groups using the DAVID tool (http://david.abcc.ncifcrf.gov/).abcc.ncifcrf.gov/
Functional clustering was performed using the DAVID database.
We analyzed the 1,817 genes that were hypermethylated only in RL cells using the DAVID interface (http://david.abcc.ncifcrf.gov/).abcc.ncifcrf.gov/
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