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We performed gene ontology and pathway analyses of the early-, middle- and late-stage HCC DMGs using the DAVID method.
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To gain a better insight on the biological processes, cellular components and molecular functions that are affected by the differentially expressed genes, we performed a functional analysis by using the DAVID toolbox (see Methods) [ 35, 36].
Statistical enrichment of functional terms in gene groups throughout this chapter were calculated using the DAVID Bioinformatics Resource [ 33] (See Methods) For a complete list of enriched categories in OCT4 targets see Additional File 3. The authors in [ 32] noted that several targets of OCT4 fall into the Wnt signalling pathway.
Listed are GO categories identified through enrichment testing of the differentially expressed genes from NHDF cells using the DAVID functional annotation system as described in the methods section.
The differentially expressed genes were functionally annotated using the DAVID integrated database query tool[ 38] and by the over-representational analysis method [ 39].
A gene ontology (GO) analysis was performed using the DAVID website.
Using the DAVID functional annotation clustering tool, the up- and down-regulated genes can be categorized into 176 and 146 overlapping functional gene clusters, respectively.
Differentially expressed genes were categorized in GeneOntology groups using the DAVID tool (http://david.abcc.ncifcrf.gov/).abcc.ncifcrf.gov/
Functional clustering was performed using the DAVID database.
We analyzed the 1,817 genes that were hypermethylated only in RL cells using the DAVID interface (http://david.abcc.ncifcrf.gov/).abcc.ncifcrf.gov/
Functional annotation and enrichment analyses were done using the DAVID platform version 6.7 (http://david.abcc.ncifcrf.gov/home.jsp) [36], [37].
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