Sentence examples for using the cycle thresholds from inspiring English sources

The relative values for each gene were determined using the cycle thresholds and normalized to reference genes.

Relative mRNA level quantifications of target genes were determined using the cycle threshold method with ATP5B and β2 microglobulin (β2M) as housekeeping genes (PrimerDesign Ltd.

The relative abundance of each target was calculated using the cycle threshold (Ct) values, using the formula 2−CT.

The calculations for determining the relative level of gene expression were made using the cycle threshold (Ct) method.

Gene expression was studied by quantitative Real-Time PCR using the cycle threshold (CT -based method [24].

Data thus obtained were analyzed by using the cycle threshold (CT) of each amplification reaction relating it to its respective standard curve.

Calculations for determining the relative levels of gene expression were made from triplicate measurements of the target gene, with normalization to β-actin in the sample, using the cycle threshold (Ct) method and the 2ΔΔct equation, as previously detailed [41].

The emission data were quantified using the cycle threshold value.

The relative quantification of gene expression was performed using the cycle threshold increment method.

Relative quantification of specific mRNA levels was analyzed using the cycle threshold (Ct) 2-ΔΔCt method.

Fold inductions were calculated using the cycle threshold ΔΔCt method as previously described.