Sentence examples for using the cycle threshold from inspiring English sources

Relative mRNA level quantifications of target genes were determined using the cycle threshold method with ATP5B and β2 microglobulin (β2M) as housekeeping genes (PrimerDesign Ltd.

The relative abundance of each target was calculated using the cycle threshold (Ct) values, using the formula 2−CT.

The calculations for determining the relative level of gene expression were made using the cycle threshold (Ct) method.

Gene expression was studied by quantitative Real-Time PCR using the cycle threshold (CT -based method [24].

Data thus obtained were analyzed by using the cycle threshold (CT) of each amplification reaction relating it to its respective standard curve.

Calculations for determining the relative levels of gene expression were made from triplicate measurements of the target gene, with normalization to β-actin in the sample, using the cycle threshold (Ct) method and the 2ΔΔct equation, as previously detailed [41].

The relative values for each gene were determined using the cycle thresholds and normalized to reference genes.

The measurement of amplified cDNA used the cycle threshold (Ct) value, which is inversely proportional to the amount of cDNA.

Gene expression was calculated using the comparative cycle threshold (2−ΔΔCt) method (using the mean cycle threshold (Ct) value for 18S rRNA and the gene of interest for each sample).

Relative expression was calculated using the comparative cycle threshold method [ 114], where delta cycle threshold of cDNA from undamaged controls was defined as 100% transcript presence.

The fluorescent product was detected at the last step of the cycle, and final mRNA levels were normalized using the comparative cycle threshold method.