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HT-29 cells were prepared and treated as described, and then the percentage of cells in each phase of the cell cycle was analyzed by flow-cytometry, using the Cycle TEST PLUS DNA Reagent Kit (BD Pharmingen, San Jose, CA, USA), which is based on the measurement of the DNA content of nuclei labeled with propidium iodide (PI), according to manufacturer's instructions.
Cells were harvested, washed once with phosphate-buffered saline (PBS), and prepared for cell cycle analysis using the "Cycle Test Plus Kit" (BD, San Jose, USA).
The cells used for the cell-cycle analysis were stained with propidium oxide (100 μg/mL) using the Cycle Test Plus DNA Reagent Kit (BD Biosciences) and were analyzed by flow cytometry (FACScan; BD Biosciences) using an instrument equipped with the CellQuest software program (BD Biosciences).
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The decay rate using the on off cycle test is 0.113 mA/(cm2 min) whereas a slightly slower decay rate of 0.096 mA/(cm2 min) is obtained for the load cycle test.
Cells were harvested and stained with propidium iodide using the BD Cycle TEST PLUS Reagent Kit (BD Biosciences, San Jose, CA, USA) according to the manufacturer's instructions.
In all of the analyses reported above for the data in Yanowitz et al. (2000), the results from vehicles tested more than once using the same test cycle, and without any additional mileage accumulated between tests, are averaged to produce a single data point (US EPA, 2002).
The proliferation of cBMSCs seeded on the pure CSH and Mg/CSH composite cements was evaluated by using cell cycle test based on flow cytometry.
DNAs were stained using a Cycle TEST PLUS DNA Reagent Kit™ (Becton Dickinson, Ontario, Canada).
Changes in the severity of diarrhoea were analysed by comparing baseline levels with those of each subsequent cycle using the Stuart Maxwell test.
For the determination of cell cycle phase distribution of DNA content, EAC cells harvested from the peritoneal cavity of un-/placebo-/calcarea carbonica-treated mice tumor-bearing mice were permeabilized and nuclear DNA was labelled with propidium iodide (PI) using Cycle TEST PLUS DNA reagent kit.
To assess the developmental state of dialectic structures the Cycles test was designed and used.
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