Sentence examples for using the cycle sequencing from inspiring English sources

PCR products were subjected to DNA sequence analysis using the Cycle sequencing kit, (Applied Biosystems, Weiterstadt, Germany).

The DNA sequencing was performed by using the cycle DNA sequencing kit (DTCS CEQ2000, Beckman Coulter) as described in detail previously [ 22].

Double-stranded PCR products, treated with ExoSAP-IT (USB) to inactivate remaining primers and dNTPs, were directly used for the cycle sequencing reaction, using dye-labeled terminators (Applied Biosystems) with amplification primers and appropriate internal primers.

Samples that showed variant migration bands were sequenced by direct sequencing using the ABI BigDye cycle sequencing kit with dye-labelled terminators.

Purified PCR products were subsequently sequenced using the ABI BigDye Terminator cycle sequencing kit v3.1 and analysed on an ABI 3730 automated sequencer (PE Applied Biosystems, Foster City, CA, www.appliedbiosystems.com).

Automated DNA sequencing was performed using the PRISM Big Dye cycle sequencing system and a model 373 sequencer (Applied Biosystems).

Subsequently, PCR products were cleaned with the Qiagen Gel Extraction kit and sequenced on an ABI 3130xl sequencer using the BigDye Cycle Sequencing kit.

Automated sequencing to identify PCR products was carried out using the PRISM BigDye™ cycle sequencing system v3.1 and ABI 3730 capillary sequencer (Applied Biosystems).

DNA sequencing was performed with both forward and reverse primers using the ABI Prism Big Dye Terminator cycle Sequencing Ready reaction kit (PE Applied Biosystems) and analysed on an ABI 377 sequencer.

Sequencing is realized on the purified products using the ABI PRISM Big Dye Terminator TM cycle sequencing Reading Reaction Kit (Applied Biosystem).

Confirmation of array-based sequencing was performed with a dye-terminator platform using the ABI Big Dye cycle sequencing kit (Applied Biosystems).