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Rather than using the cut off value recommended by the manufacturer, we estimated a new cut off value from the TG-ROC analysis to optimize the discrimination between positive and negative results [ 19].
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The association between allergens and asthma was also analyzed by using the cut off values of 1 and 8 μg/g of cat allergen per gram of dust.
In our study, we used the cut off value of >8% to identify patients with unacceptable glycaemic control.
Using the cut-off value of 30 U/L, the overall sensitivity, specificity, positive likelihood ratio, and negative likelihood ratio of ADA was 94%, 95%, 19, and 0.06 respectively.
Using the cut-off value ≤19, sensitivity was 83% and specificity was 96%.
The significance of differential expression was determined using the cut-off value of p ≤ 0.00056.
The PFS and OS rates were also analyzed using the cut-off value from ROC analysis.
We define lipodystrophy by FMR using the cut-off value of 1.961 for men and 1.329 for women [ 10].
Hence, we divided the MPD and MNTD coordinates into 4 quadrants using the cut-off value -2.
The results were almost the same; however, the P-value was smaller when using the cut-off value of 75%.
No significant difference was observed using the cut-off value as suggested by the manufacture (< 0.35 = negative, > 0.35 = positive).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com