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Aconitase activity was measured by monitoring conversion of citrate into α-ketoglutarate at 340 nm at 25°C using the coupled reduction of NADP to NADPH [48].
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Mitochondrial citrate synthase activity was measured by the reduction of DTNB [5,5′-dithiobis- 2-nitrobenzoic acid)] by citrate synthase and using the coupled reaction with acetyl-CoA and oxaloacetate [5,5′-dithiobis- 2-nitrobenzoic
By using the coupled model, an important reduction in the number of degrees of freedom and in the computation time compared to that needed for the discrete approach, is observed.
Intracellular proteins were extracted from mycelium cells and investigated for AfAGM1 activity using the AfUAP1 coupled assay revealing a 50% reduction in AGM1 activity.
We measured PDI activity in the protein extracts from cells overexpressing PDI with and without Txnip using a coupled insulin reduction assay.
Realign bones using the open reduction method.
Realign bones using the closed reduction method.
CeO2 is added to glass containers to protect the product from deterioration due to long-term exposure to ultraviolet radiation from sunlight, again using the Ce4+-Ce3+ oxidation-reduction couple.
A 3-dimensional benchmark simulation was conducted with a 750 t/day commercial incinerator using the present coupling scheme incorporating with a reduced SNCR reduction mechanism.
To demonstrate the utility of OYE2p for practical applications, the asymmetric reduction of (E/Z -citral on a gram scalE/Z -citralrmed using the reconbinant OYE2p coupled with gramoscalehydrogenase (BsGDH) from Bacillus megaterium that was used to regenerate NADH.
Using the entropy concept analytical reduction to a pressure velocity coupling is found.
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