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The antioxidant activity of the extract fractions was determined using the coupled oxidation of linolenic acid and β-carotene.
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In the coupled oxidation reaction, the final product, verdoheme, can readily be converted to biliverdin under hydrolytic conditions.
Analysis of the coupled oxidation of cytochrome b5 variants revealed critical differences between enzymatic and nonenzymatic heme degradation.
The iron complex of (the symmetric) octaethylporphyrin underwent coupled oxidation to give a bilin-type tetrapyrrole with good yield.
Coupled oxidation of the H63V variant could be inhibited by the addition of the H2O2-scavenging enzyme catalase.
'Coupled oxidation' of heme (or of its dimethyl ester) with ascorbate and oxygen was studied as a model reaction for the oxidation of heme to BV by HO.
The H2O2 released from BAT mitochondria was determined fluorometrically using the Amplex Red oxidation method coupled to the enzymatic reduction of H2O2 by HRP (horseradish peroxidase), as previously described [ 20].
CeO2 is added to glass containers to protect the product from deterioration due to long-term exposure to ultraviolet radiation from sunlight, again using the Ce4+-Ce3+ oxidation-reduction couple.
A typical galvanic cell system with two half-cells (A and B) was used to generate a current by coupling oxidation and reduction reactions in a spontaneous process, in which the A cell solution is 5 15 mM ZnSO4 and the B cell solution is 0.25 M NaOH.
Rapid Fe(II) oxidation in the presence of organic ligands did not necessarily result in the coupled As III) oxidation.
Based on experimental observations, and model calculations using a coupled thermodynamic-kinetic oxidation model, four successive growth stages can be identified for the oxidation of a γ-Ni 27Cr 9Al alloy at 1373 K.
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