Sentence examples for using the coupled enzyme from inspiring English sources

OGT assay using the coupled enzyme method: 1.

Using the coupled enzyme method, it was easy to evaluate the C-654 activity by continuous measurement of OD570 nm.

It was found that OD570 nm also rose linearly with increasing UDP concentration, indicating that UDP could be analyzed quantitatively using the coupled enzyme method.

Nevertheless, the increase of PmHS2-GlcUA+ concentration resulted in the synthesis of a higher number of heparosan chains and higher polymerization efficiency, as observed using the coupled enzyme assay (data not shown).

JMJD2E-catalyzed (100 nM) demethylation of a trimethylated histone H3 peptide was measured in the presence of the potential inhibitor (3.7 nM–57 µM) as reported [18] in a fluorescence-based enzymatic assay using the coupling enzyme formaldehyde dehydrogenase (FDH) [20].

To allow continuous monitoring of Dam methylation, hemimethylated oligonucleotide 1 (Fig. 1) was used in the coupled enzyme assay.

The kcat of the coupled enzyme system using chromopyrrolic acid 1 is 0.068 ± 0.01 s−1.

Low-activity enzymes were characterized using the discontinuous assay; high-activity ManDs were characterized using the coupled assay.

ATPase rates were determined at 23 °C in HKM buffer (50 mM Hepes, pH 7.3, 150 mM KCl, 10 mM MgCl2) containing 1 mM ATP by measuring phosphate released using the EnzCheck coupled enzyme phosphate assay kit as described previously (16).

Activity was quantified using a coupled enzyme assay, monitoring the NADH-dependent reduction of acetaldehyde by alcohol dehydrogenase, as described previously (21, 22).

The variants were screened using a coupled enzyme assay for their ability to cleave (unphosphorylated) 2-keto-3-deoxygluconate (24): in this assay, 1% of the variants were more active than the wild-type enzyme.