Sentence examples for using the couple of from inspiring English sources
Using the couple of primers specific for both minigene and Tim44, no signal could be detected correctly from cells transfected with the empty vector.
DNA extraction was achieved as described by Liu et al. [ 18]; the amplification of the internal transcribed spacer regions (nuclear-encoded 18S rRNA-ITS1-5.8S rRNA-ITS2-28S rRNA) was performed using the couple of universal primers ITS1 (5′-TCC GGT GAA GAA CCT GCG G-3′) and ITS4 (5′-TCC TCC GCT TAT TGA TAT GC-3′) [ 19] and the thermal cycler conditions according to Luo and Mitchell [ 20].
Using the coupling-of-modes (COM) analysis, the device was simulated, and the effect of reflector configuration and interdigital transducer (IDT) structure on the performance of the devices was studied.
The present study applies an evolutionary approach for determining the optimal geometry of 2D structures using the coupling of the boundary element method (BEM) and the level set method (LSM).
Self-powered characteristic of the UMS is achieved based on the principle of triboelectric nanogenerator by using the coupling of triboelectrification and electrostatic induction as governed by the Maxwell's displacement current.
The antioxidant activity of the extract fractions was determined using the coupled oxidation of linolenic acid and β-carotene.
Aconitase activity was measured by monitoring conversion of citrate into α-ketoglutarate at 340 nm at 25°C using the coupled reduction of NADP to NADPH [48].
The analysis takes into account the effect of propagation using the coupled transmission lines theory.
Here we describe adamantyl arotinoids with 2,2′-disubstituted biaryl rings prepared using the Suzuki coupling of the corresponding fragments.
The 5-OH-CB77 and 6-OH-CB77 metabolites were prepared using the Cadogan coupling of 3,4-dichloroaniline with 2,3-dichloroanisole and subsequent demethylation with boron tribromide (20).
Plasmids carrying the same point mutations described above were generated by using the same couples of primers, and amplified into TOP10 competent cells (Life Technologies).
