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The absolute amounts of each miRNA, per μl of serum in each patient sample were determined as above using the corresponding synthetic miRNA to generate the standard curve.
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Nine suitable peptide sequences from extracellular regions of these eight proteins were identified and used to guide the manufacture of the corresponding synthetic peptides.
Immunoreactivity was competitively inhibited by addition of 10 μg of the corresponding synthetic peptides used to generate Abs.
The specificity of the immunolabeling was checked by pre-adsorption of anti-PdC-Lectin and anti-Pdcyst-rich protein IgG with the corresponding synthetic peptides used for immunizations.
An artist designer interpreted several vocal self-portraits and rendered the corresponding synthetic sketches by using physics-based and concatenative sound synthesis.
The new prediction equation was obtained by regressing the obtained testcross phenotypes on the corresponding synthetic seed genotypes collected within a cycle using ridge regression as in the discovery phase.
To assess the possible role of this bi-helix in IN we carried out an analysis of the structural and binding properties of the corresponding synthetic peptide (residues Gly-149 to Lys-186) using circular dichroism (CD) [43] and fluorescence.
The corresponding synthetic gene, codon-optimized for E. coli expression was obtained from Geneart (Regensburg, Germany), and used to express two variants of the encoded recombinant antigen.
Therefore, the corresponding synthetic gene was ordered as an E. coli codon-optimized variant (Genscript).
We describe the conformational and binding properties of the corresponding synthetic peptide.
Two target RNAs were synthesized with T7 RNA polymerase (Promega) from the corresponding synthetic DNA templates.
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