Controls were performed by omitting the primary antibody and by using the corresponding non-specific IgG fraction.
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TRITC-conjugated swine anti-rabbit (1/40 dilution in PBS, Dako, Glostrup, Denmark) and Alexa Fluor 680-labeled goat anti-rat (1/50 dilution in PBS, Invitrogen) secondary antibodies, as appropriate, were used to fluorescently label the corresponding primary antibodies.
Using this signature, the analysis reported an even closer correlation between orthoxenografts and the corresponding primary tumors (R ~0.95); by contrast, using the same signature, lower correlation values were reported between distinct primary tumors (R ~0.78) and between primary tumors and non-corresponding xenografts (R ~0.8) (Fig 6A).
Horseradish peroxidase-labelled secondary antibodies (GE Healthcare, Waukesha, WI, USA) against the corresponding primary antibodies were used.
We used the positional information of the secondary structure along with the corresponding primary structure nucleotide, yet we were able to achieve a high sensitivity and specificity.
Negative controls, prepared by omitting the corresponding primary antibody and adding the secondary immunoglobulin G (IgG) isotype antibody, were also used in each separate experiment.
The resonant waves with difference frequencies propagate in the opposite direction of the corresponding primary wave.
The corresponding primary tumor, presumably an ILC, remained uncharacterized [ 28].
Detection was performed using a LiCor Odyssey instrument, using the indicated primary antisera with corresponding LiCor secondary antibodies.
