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This was performed by EIA assays using the corresponding monoclonal antibodies (Mabs) against individual HBGAs as described previously (Huang et al., 2005; Tan et al., 2008).
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After generation of numerous hybridomas, the corresponding monoclonal antibodies were subjected to primary screening using cell cultures and human tissues.
The corresponding monoclonal antibody H5N1 HA1 (clone 4E10C10) was also aquired from ProSci Incorporated.
The supernatant was collected and analyzed by western blot using the 4B7 monoclonal antibody (mAb).
Immunofluorescent analysis was performed as described previously using the same monoclonal antibody18.
A different picture emerged using the mouse monoclonal antibody against lipopolysaccharide.
The RPCI staining was performed using the rabbit monoclonal antibody.
Less than 10% labeled using the D1.1 monoclonal antibody.
The intra- or extracytoplasmic nature of HA tags was determined for each construct by immunofluorescence with their corresponding LLC-PK1 transfectants using the anti-HA monoclonal antibody.
Osteogenic differentiation was evaluated using the following antibodies: monoclonal BAP US Biologicall, USA), mouse anti-human, monoclonal BSP mouse anti-human (Chenicon International, Prodotti Gianni, Milan, Italy), monoclonal osteonectin mouse anti-human (Novocastra Laboratories, Ltd ,UK) and polyclonal osteocalcin (Santa Cruz, CA, USA) goat anti-human.
PGK was detected using the monoclonal antibody 22C5 (Molecular Probes).
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