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Approximately 1000 CFU were pre-screened using the corresponding agar plate detection systems for all three expression systems.
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The corresponding agar plates revealed massive bacterial growth far above 1000 CFU.
Aeromonas hydrophila,Pseudomonas aeruginosa, and total and faecal coliforms were enumerated by the membrane filtration method (0.45 mm, Gelman Science, Ann Arbor, MI, USA) using the Aeromonas agar, Pseudomonas agar base (Oxoid, Hampshire, Eng) and ECC agar (Chromagar, Paris, France), respectively.
Denitrifying bacteria was isolated from the incubated soil using the standard agar plate dilution (APD) method.
Then, the culture was subjected to separation using the LB agar plate to obtain single clones.
Coliphages were detected using the double agar layer method.
The antibacterial activity of the novel candidates has been screened using the agar diffusion test.
Using the agar spot method, ten out of the 21 tested bacteria showed antilisterial activity.
Antimicrobial assay: The antimicrobial activities were determined using the agar diffusion technique in Petri dishes.
The tests were performed using the agar medium assay described by Tatsadjieu et al. [20].
Each dilution was titrated using the agar overlay method.
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