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We can generally expect more accurate solutions by using the incorporation approach described in Li et al. (2008), because inversion solutions obtained using the correction approach depend on the accuracy of the approximation of the sub-seafloor structure.
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The prevalence of vitamin A deficiency was 16.3% using the correction factor approach and 12.7% if children with inflammation were excluded.
The prevalence of iron deficiency was similar using the correction factor approach or by excluding children with inflammation and measuring the prevalence of iron deficiency in the remaining children (34.6% versus 35.1%, respectively).
Adjustments for multiple comparisons were made using the Bonferroni correction approach [ 48].
However, daily predictions of intakes based on a single urine void using the creatinine-correction approach showed wide variability.
At least for this individual and this cohort, urine samples taken during the day and used to extrapolate intakes using the creatinine-correction approach would result in an underestimate of overall intakes.
The average model-based daily intake for this participant (18.3 µg/kg-day) was similar to the average daily intake calculated using the creatinine-correction approach (19.4 µg/kg-day) (see Supplemental Material, Table S2).
However, using the k-correction approach on non-normalized RAS values, one would predict an allelic frequency of 90%, whereas the actual frequency is 100%, a bias of 10%.
As mentioned in the introduction, the previous suffix tree based error correction approaches use the correction for a read that is first encountered during tree traversal and neither store all possible corrections nor choose the optimal one.
The correction approach was carried out using data from both strands of DNA in all cases.
Background-subtracted data were log2-transformed and normalized using quantile correction approach.
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