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The amount of protein carbonyls in the sample was calculated using the corrected absorbance for each sample and an extinction coefficient of 2.2 × 10 M-1 cm-1.
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The equation was then used to convert the corrected absorbance readings of the single working dilution directly into the predicted ELISA antibody titres.
The relative frequency was calculated based on the corrected absorbance using the following formula: relative frequency = (corrected absorbance of TCRV-specific probe/sum of corrected absorbance of TCRV-specific probes) × 100.
The corrected absorbance of each sample was calculated by comparison with the untreated control.
If the corrected absorbance does not exceed the limits prescribed in the Characteristics the substance meets the ultraviolet absorbance specifications.
The corrected absorbance was calculated by subtracting the absorbance of the well containing the control tube aliquot from the absorbance of the well containing the sample tube aliquot.
The corrected absorbance of each sample was calculated and compared with that of the untreated control.
The method corrects the specimen's absorbance at 620 nm for the absorbance of contaminating heme pigments, using the following formula: corrected absorbance at 620 = actual absorbance at 620 nm - [1.426 (absorbance at 740) + 0.03].
The method corrects the specimen's absorbance at 620 nm for the absorbance of contaminating heme pigments by means of the following formula: corrected absorbance at 620 = actual absorbance at 620 nm - (1.426 [absorbance at 740 nm] + 0.03).
All absorbance measurements were corrected to the reference wavelength (Corrected absorbance = 570 650 nm).
The reading at 690 nm was used as a reference wavelength to calculate a corrected absorbance (A550 – A550).
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