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The calculated 2−ΔΔCt was transformed into a percentage using the control value as 100% to indicate the mRNA expression.
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When APE1 activity values were divided into tertiles using the controls' values, the adjusted odds ratio for the lowest tertile versus the highest was 3.3 (95% CI = 1.4 8.1; P = 0.008).
Then, data were filtered using the control strength, a control value calculated using the Cross-Gene Error Model on replicates [ 14] and based on average base/proportional value.
Then data were filtered using the control strength, a control value calculated using the Cross-Gene Error Model based on replicates.
We observed that, on average, the eGFP variants generated using the high expression matrix are statistically better expressed than those variants generated using the control matrix (p-value = 6.1e-6) and better than the wild-type eGFP (p-value = 1.4e-6).
The RTA mRNA level was then normalized to the β-actin mRNA level, and the relative quantity of RTA mRNA in 293/Bac36 cells transfected with pCDH-GFP was used as the control value.
Subsequently samples were corrected for loading using the control band and finally values were expressed relative, defining the intensity of the wild type sample as 1.
In comparative transport assays using a substrate mixture the control value was used to normalize the transport rates.
While using the inflated control value may reduce the sensitivity for gastroparesis, it will increase the chance to discover other major diseases that might be causing similar clinical symptoms.
This curve was used to adjust the control value for each measurement.
Densitometry was carried out using Image J software and statistically analysed using factorial analysis of variance (ANOVA) using STATVIEW v4.53 (Abacus Concepts) where the control value is set to 100%.
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