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Since our methodology normalizes using the control line, general illumination changes as caused by placing multiple tests farther from the center of the imaging area (see Figure 6 a,b) do not significantly alter test results compared to those from single RDT images for the same tests.
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And, of course, you can also enable SSH access to control it using the command line or even an SSH app on your phone.
Male w1118 Canton-S B flies were used as the control line.
We also generated p65ADZp and ZpGAL4DBD lines using the control regions from the genes encoding the enzymes for synthesizing monoamine neurotransmitters: tyrosine hydroxylase, dopamine decarboxylase, tryptophan hydroxylase, and tyrosine decarboxylase.
If you want to use the command line, click on Control Panel.
In addition, a UBE3A knockout (KO) line was generated from one of the control lines using CRISPR/Cas9-mediated gene editing (see Methods).
The oscilloscope was used to test out if the control lines coded enabled or disabled clock signals at the right moment to provide the pulses or waveform required by the CCD sensor or AD9822.
The consequences of using a control line are evaluated under two scenarios.
Compared to the experimental control using the cell line, a higher background was observed in clinical samples (Fig. 4b, upper panels).
One can linearly modulate the effective LED intensity by varying the time that a flickering LED spends in the on state relative to the off state, using a digital control line.
This was resolved by using control line estimates of the resident seed bank, estimates of the proportion of sown seed that was truly hybrid and measures of B. rapa vital rates from the relevant experimental lines in a series of equations to calculate true hybrid vital rates using least squares estimation (Supplementary Data Methods S2).
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