(C) PC3 cells transfected transiently and stably with GFP-EN2 plasmid DNA were then co-stained with wheat germ agglutinin membrane stain (red), these were imaged and analysed using the confocal microscopy software to show co-localisation (40X magnification), the arrows on the images depict the cell through which the measurement was taken.
Three-dimensional image rotations were derived from confocal image stocks using the confocal system software.
Time series were captured and processed using the confocal LAS AF software (Leica).
Fluorescent confocal laser scanning microscopy was conducted with a Leica SP2 MP laser scanning confocal microscope using the Leica confocal software LCS Lite Leica Microsystems HD GmbHH, Mannheim, Baden-Württemberg, Germany).
Images were acquired with Leica TCS SL confocal system using the Leica confocal software (Leica Microsystems, Wetzlar, Germany).
Post-capture image analysis and processing of confocal image stacks were performed using the Leica Confocal software.
Images were acquired with a Leica TCS SP2 confocal system (Leica Microsystems Heidelberg, Mannheim, Germany) using the Leica Confocal Software (LCS).
Moreover, a quantitative analysis, carried out using the Leica confocal software, allows us to quantify the fluorescence intensity increase of LC3 stained cells exposed to simulated microgravity and 6(c)).
Image acquisition and processing were achieved using the Leica Confocal Software (LCS) (Leica Lasertechnik, Heidelberg, Germany) and Adobe Photoshop software programs (Adobe system, Mountain View, CA).
Image acquisition and processing were carried out using the Leica Confocal Software (Leica Lasertechnik).
