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The localized expression of AQPs in the plasma membranes can be more clearly observed using the confocal fluorescence microscope (see the Supporting Information).
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The composition of these "rivets" has been examined immunocytochemically and in three dimensions using the confocal laser scanning epi-fluorescence microscope.
Furthermore, the confocal fluorescence imaging using HeLa cells showed that the probe could be applied to detect Fe3+ in living cells.
The images were examined using a confocal fluorescence microscope (Nikon) and SPOT imaging software (Diagnostic Instruments, Inc., Sterling Heights, MI, USA).
Finally, the cells were analyzed using a confocal fluorescence microscope (Olympus FV-1000; sOlympus, Olympus FluoView; Olympus, Center Valley, PA, USA).
DAPI (4′-6-diamidino-2-phenylindole) was used to stain cellular nuclei, and the cells were observed for epifluorescence using a confocal fluorescence microscope (Leica).
Using scanning confocal fluorescence microscopy, single emitters on resonance with a single mode of the microresonator can be spatially addressed.
Images were obtained using confocal fluorescence microscopy using step-wise 1 μM z-stack sampling.
The samples were then subjected to electrophoresis, stained with DAPI and observed and photographed using a confocal fluorescence microscope.
The slides were then mounted in a mounting medium (Vector Laboratories) and visualized and photographed using a confocal fluorescence microscope (LSM 710, Carl Zeiss International).
Using a confocal fluorescence microscope (Zeiss 510 META), fluorescence images of CXCR4CFP and Fluo-4 were simultaneously recorded in a time-lapse experiment (Figure 2A).
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