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Confirmatory factor analysis was performed on both OLQ-13 and OLQ-11 using the confirmation set.
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We used our PBC analysis pipeline to classify the 12 new human miRNAs in this release by treating them as the confirmation set.
Since these primers gave occasional false-positive reactions with stool specimens, all PCR-positive stool specimens were retested by the LightCycler PCR (Roche Diagnostics GmbH, Mannheim, Germany) for confirmation using the same two sets of primers, with the melting curve analysis being used to provide additional confirmation of reaction specificity (9 ).
Hence, we first confirmed bimodality in the separate clusters before using the entire data set for further confirmation and association analyses.
(Use the "warm" setting).
Use the Groups setting.
Use the cool setting.
Otherwise, use the "sun" setting.
To confirm the differential expression profiles, we further examined the expression levels in murine fundic mRNA derived from wild types and W/W V mice maintained under fasted conditions as templates, using semi-quantitative RT-PCR analysis (Representative data were shown in Fig. 1C,1D. All primer sets used for the confirmation were listed in Table 1).
Tables 9, 10 show the results of the confirmation experiment using the optimal operating parameters.
Confirmation using the coronal plane might become redundant when using 3D modelling.
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