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Conjugation experiments showed that five of 17 tet(A) positive bacteria successfully transferred the gene to the recipient host using the conditions employed in this study (three Alcaligenes and two E. coli; Table 5).
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When post-therapy sequences could not be obtained using the conditions previously employed for the pre-therapy samples [32], pre-therapy samples were resequenced using the primers and conditions that were used to amplify the post-therapy samples.
The L72V, A236G, W325F, and V329A EIZS mutants were crystallized by the hanging drop vapor diffusion method using the same conditions employed for crystallization of the wild-type enzyme.
No H2 gas was detected in the culture headspace from both batch and continuous culture using gas chromatography suggesting that M. smegmatis did not produce detectable levels of H2 using the culture conditions employed in this study.
Human CSE did not generate detectable H2S levels under the conditions employed when homocysteine was used as a substrate (data not shown).
We conclude that under the conditions employed in our experiments, and using a highly-sensitive assay, we could not detect any consequence of RF exposure.
The conditions employed for enzymatic hydrolysis step (incubation temperature, time and pH) were optimised using 3′-hydroxystanozolol glucuronide.
with the conditions employed in this study.
was employed for CFD analysis, using the conditions shown in Table 2.
Therefore, the optimal DLR appears to be in the range of 4-10 when using conditions employed in the present study.
Using the condition (H) together with the method employed in Step 1 of the proof of Lemma 2.3, we find that.
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